Reverse Pipetting; Storage; Testing The Performance And Recalibration; Performance Test (Checking Calibration) - Biohit Proline Mode D'emploi

6.2. Reverse pipetting

A selected volume plus an excess is aspirated into the tip. The delivery
is done without blow-out, and so the excess volume remains in the tip.
The reverse technique is recommended for high viscosity, biological or
foaming liquids, or very small volumes of liquid.
1. Depress the operating button all the way to the second stop
(Fig. 9C). Place the tip(s) just under the surface of the liquid
(2-3 mm) and smoothly release the operating button up to the
starting position (Fig.9A).
2. Withdraw the tip(s) from the liquid touching against the edge of
the container to remove excess.
3. Deliver the preset volume by smoothly depressing the
operating button to the first stop (Fig. 9B). Hold the operating
button at the first stop. The liquid that remains in the tip(s)
should not be included in the delivery.
4. The remaining liquid should now be discarded with the tip(s) or
delivered back into the container vessel.
7.

Storage

When not in use it is recommended that your Biohit Proline pipettor is
stored in a vertical position. See Installing the pipettor holder (Chapter
3).
8.

Testing the performance and recalibration

It is recommended to check the performance of your Biohit Proline
pipettors regularly (e.g. every 3 months) and always after in-house
maintenance. However, the user should establish a regular testing
routine for their pipettors with regard to accuracy requirements of the
application, frequency of use, number of operators using the pipettor,
nature of the liquid dispensed and the acceptable maximum
permissible errors established by the user. (ISO 8655-1.)
8.1. Testing the performance
Performance testing should take place in a draught-free room at 15 –
30 O C, constant to +/- 0.5
and the test water should have stood in the test room a sufficient time
(at least 2 hours) to reach equilibrium with the room conditions. Use
distilled or deionised water (grade 3, ISO 3696). Use an analytical
balance with a readability of 0.01 mgs. (ISO 8655-6.)
Weighing
1. Adjust the desired test volume V
2. Carefully fit the tip onto the tip cone.
3. Fill the tip with test water and expel to waste five times to reach a
humidity equilibrium in the dead air volume.
4. Replace the tip. Pre-wet the tip by filling it once with test water and
expel to waste.
5. Aspirate the test water, immersing the tip only 2-3 mm below the
surface of the water. Keep the pipettor vertical.
6. Withdraw the pipettor vertically and touch the tip against the inside
wall of the test water container.
O C and humidity above 50%. The pipettor, tips
.
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