Pipetting Techniques; Forward Pipetting - Biohit Proline Mode D'emploi

6.

Pipetting techniques

1. Make sure that the pipettor, tips and the liquid are at the same
temperature.
2. Make sure that the tip is firmly attached to the tip cone.
3. Hold the pipettor vertically when aspirating the liquid and place
the tip only a few millimetres into the liquid.
4. Prerinse the tip before aspirating the liquid by filling and
emptying the tip 5 times. This is important especially when
dispensing liquids which have a viscosity and density different
from water.
5. Always control the operating button movements with the thumb
to ensure consistency.

6.1. Forward pipetting

This technique employs the blow-out function ensuring complete
delivery of the liquid.
1. Depress the operating button to the first stop (Fig. 9B).
2. Place the tip(s) just under the surface of the liquid (2-3 mm) and
smoothly release the operating button up to the starting
position (Fig.9A). Carefully withdraw the tip from the liquid,
touching against the edge of the container to remove excess.
3. Liquid is dispensed by gently depressing the operating button
to the first stop (Fig. 9B). After a short delay continue to
depress the operating button to the second stop (= blow-out)
(Fig 9C). This procedure will empty the tip(s) and ensure
accurate delivery.
4. Release the operating button to the starting position (Fig. 9A).
If necessary change the tip(s) and continue pipetting.
Starting position
Fig. 9A
First stop
Fig. 9B
9
Second stop
Fig. 9C