8 | Red Sea | Reef Care Program
Directions for Phosphate Pro Test Kit
1.
Using the syringe provided, place exactly 17 ml of the water to be tested into both
of the glass vials.
Insert one of the vials (control vial) into the outer hole of the comparator.
2.
Add 13 drops of Phosphate Pro Reagent A to the other vial (reaction vial), close the
3.
vial with the cap and shake for a few seconds.
4.
Add 4 drops of Phosphate Pro Reagent B using the pipette, close the vial with the cap
and shake for a few seconds.
5.
Remove the cap from the reaction vial and insert the vial into the center hole of the
comparator.
6.
Wait 15 minutes for the color in the reaction vial to stabilize.
NOTE: If the water sample has cooled to below 20°C wait for 20 minutes.
When the end point of the test reaction has been reached look into both vials from
7.
above and rotate the color disc until the closest color match possible is achieved
between the vials. Note: Turbidity or severe coloration of the water sample may
distort the colors.
8.
The phosphate level corresponding to the color selected is printed on the color disc as
indicated by the pointer on the side of the comparator body. If necessary, estimate an
intermediate value.
The color in the reaction vial will remain stable for 5 minutes. Do not relate to the
9.
color in the reaction vial after this time.
High Range: For levels of phosphate above 1 ppm dilute 1ml of the water to be tested
with 16ml of RO water. Multiply the test result by 17.