Bresser Erudit MO Mode D'emploi page 11

1. Stage Installation
The microscope's slide stage (Fig. 1, 13) is simple to attach.
There are two small pegs on the bottom of the slide stage that
fit directly into the holes on the microscope stage (Fig. 1, 7).
The stage retaining screw (Fig. 1, 14) attaches the slide stage
to the microscope stage.
2. Electronic Illumination
The Erudit is equipped with battery-operated LED lighting. This
allows you to use your microscope without a power supply.
The microscope is operated with the mains adapter provided
(Fig. 10, 8) to charge the battery or during use at home. To do
so, connect the power cable to the microscope's power con-
nection (Fig. 5, 22) and the adapter to a power point.
Press the on/off switch (Fig. 6, 20) to activate. The brightness
of the lighting can be adjusted with the brightness control
(Fig. 6, 11). The batteries begin charging after the microscope
is connected to the power supply. The status LED turns red
(Fig. 5, 21). When the battery is charged, the Status LED
changes from red to green (Fig. 5, 21). Now you can use the
microscope without the power supply.
The LED lighting lasts up to 48 hours when in battery opera-
tion. It can last longer, depending on the intensity of brightness
used.
3. Microscope setup
The microscope's eyepiece (Fig. 1, 5) will now be prepared for
the first observation. First, rotate the eyepiece into a conve-
nient position.
Begin every observation with the lowest magnification.
Place the microscope's table (Fig. 1, 7) with the focus wheel
(Fig. 1, 8) into the lowest position and rotate the objective
revolver (Fig. 1, 6) until it locks on the lowest magnification
(4x)
Insert the 5x eyepiece (Fig. 9, 3) in the Barlow lens (Fig. 9, 6).
Take care, that the Barlow lens is inserted completely in the
eyepiece holder (Fig. 1, 3).
4. Observation
After you have set up the microscope with the corresponding
illumination, the following principles are important:
Begin each observation with the lowest magnification, so that
centring and positioning as well as focussing of the object to
be viewed is easier.
The higher the magnification, the more light is required for
good picture quality.
Begin with a simple observation.
With the mechanical desk (Fig. 1, 13) you can position your
specimen (Fig. 3, 19) smoothly and very precise.
The object is placed between the clamps on the mechanical
desk.
Move the object, with help of the adjustment knobs, (Fig. 3,
15/16) to centre it under the objective.
With the built in vernier (Fig. 3, 17) at both axes you can now
specifically set and shift the object. It can now be viewed with
different magnifications.
Look through the eyepiece (Fig. 1, 1) and turn carefully the
focusing wheel (Fig. 1, 8) until you can see a sharp picture.
Now you can get a higher magnification, while you slowly pull
the Barlow lens (Fig. 4, 2) out of the eyepiece holder (Fig. 4, 3).
If the Barlow lens is in the most outward position, the overall
magnification of the microscope is multiplied by a factor of
1,6x.
For still higher magnification you can put the 16x eyepiece
(Fig. 9, 5) into the eyepiece holder and and set the objective
revolver on higher magnification (10x / 60x). In this setting the
overall magnification may also be multiplied by a factor of 1,6x
by pulling out the Barlow lens.
Filter wheel:
The filter wheel (Fig. 1, 9) enables you to change the colour fil-
ter to improve the contrast of your specimens. The filter wheel
has five different colour filters and one filter-free setting.
TIP:
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Depending on the preparation higher magnifi-
cations do not always lead to better pictures.
Please notice:
When changing magnification (eyepiece or objective lens
changes, pulling out of the Barlow lens) the sharpness of
the image must be newly defined by turning the focusing
wheel (Fig. 1, 8).
NOTE:
Please be very careful when doing this. When
you move the mechanical plate upwards to fast
the objective lens and the slide can touch and
get damaged.
5. Viewed Object – condition and preparation
Condition
With an ordinary magnifying glass, we preferably view obscure
items like little animals, plants etc. Here light shines on the
viewed item, gets reflected and gets through the lens into our
eye (Top illumination principle). With a biological microscope,
a so called transmitted light microscope, only transparent
items can be observed. Light gets through the item, becomes
magnified by the objective and the eyepiece and gets in our
eye. Many small water organisms, small plant parts (e. g. moss
leaves) and finest animal components are naturally transpar-
ent; other ones must be accordingly prepared. By means of
a pre-treatment or penetration with suitable materials (media)
some specimens can be rendered transparent or we cut finest
slices off of them (hand cut, Microtome cut) and then examine
these. The following instructions make us familiar with these
methods.
Preparing a thin cut of a specimen
As already before mentioned, very thin cuts have to be pre-
pared from a non transparent object (plant rods, roots, etc.). In
order to get satisfying results, we need some wax or paraffin
to coat and harden the specimen if it is soft. If no such mate-
rial is contained in your microscope set, then you can use a
normal candle. The wax is given to a pot and melted over a
flame. The object is then dipped several times into the liquid
wax. Let the wax become hard. With a microtome or a knife
/ scalpel (caution!!!) thin slices are cut off of the object coated
with wax. These cuts are put on a glass slide and covered with
a cover glass.
Manufacture of an own preparation
Put the object which shall be observed on a glass slide and
pipette a drop of distilled water on the object. Set a cover
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