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Procedure
1.
Place 5-6 drops of clean water (tap, distilled, or deionized) into the sponge inside the
calibration bottle. Turn the bottle over and allow any excess water to drain out of the bottle.
The wet sponge creates a 100% water-saturated air environment for the probe, which is
ideal for calibration, transport, and storage of the Model DO200 probe. For calibration, the
probe remains in a water saturated air atmosphere and is not submersed.
2.
Slide the probe into the calibration bottle. Be sure the membrane does not touch the
sponge.
3.
Turn on the unit by pressing
temperature readings to stabilize.
4.
Press CAL.
The LCD prompts for the local pressure in mBar. Use the ∆ ∆ ∆ ∆ and ∇ ∇ ∇ ∇ keys to increase or
5.
decrease the pressure value respectively. See the section titled 'Conversions' to convert
barometric pressure units to mBars.
6.
When the proper pressure displays, press
right of the display. Once the value in the main display stabilizes, press
the salinity compensation procedure.
The display prompts for the approximate salinity of the water to be analyzed. Use the ∆ ∆ ∆ ∆ and
7.
∇ ∇ ∇ ∇ keys to increase or decrease the salinity compensation value to the value of your sample
(between 0 to 40 parts per thousand [ppt]). When the correct salinity displays, press
8.
The unit holds calibration even if it is powered off. However, it is recommended to check
calibration with each use and recalibrate as necessary to prevent drift. Dissolved oxygen
readings are only as good as the calibration.
PROBE MAINTENANCE
To clean the probe, use the YSI Probe Reconditioning kit (part number 5238). In addition to the
Reconditioning Kit, you may try a chemical cleaning. To clean the electrodes chemically,
perform an ammonium hydroxide soak.
1. Remove membrane cap and rinse the probe with clean water (tap, distilled, or deionized).
2.
Turn unit off, or disconnect probe.
3. Obtain either:
• 14 % lab strength ammonium hydroxide and soak for 2-3 minutes
• 3% household cleaning strength ammonia and soak overnight (8-12 hours)
4.
Rinse ammonium hydroxide/ammonia from probe.
5.
Use sandpaper (400 grit wet/dry, supplied with 5238 kit) to buff (wet sand) excess deposits
from probe.
6.
Install a new membrane cap.
Never use chemicals or abrasives not recommended by YSI.
. Wait 10 to 15 minutes for the dissolved oxygen and
once to view the calibration value in the lower
5
again to move to
.
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