HTL DISCOVERY Comfort Mode D'emploi page 9

Press the pipette tip firmly.
• The shaft nut is loose (Fig. 3F) in the models DV/D2-
DV/D1000
Tighten the shaft nut.
• The sealing surface of the shaft is cracked or scored.
Remove the tip ejector. Unscrew the shaft nut, inspect
the shaft and the piston assembly. Replace the dam-
aged parts (see sec tion 12). When reassembling the
pipette, the nut should be hand tightened.
In the models DV/D2, DV/D10 and DV/D20, the damage
of the shaft may also cause a damage of the piston
assembly. Replace the damaged parts (see section 12).
When reassembling the pipette, the nut should be
hand tightened.
To remove the tip ejector in models DV/D5000 and
DV/D10000, remove the ejector pushbutton (Fig. 3N)
and using a screw driver unscrew the tip ejector by
turning the screwdriver counter-clockwise.
• Damage to the piston or seal due to prolon ged use
with the aggressive liquids.
Disassemble the pipette as described above.
Replace the piston, seal and O-ring (see section 12).
Rinse the inside of the shaft in distilled water and dry.
Lubricate the seal and O-ring with the lub ri cant, that
has been included with each pipette.
The replacement of the piston requires con duc ting
of calibration procedure.
Note: The parts of DV2/D2 and DV/D10 pipette
should be lub ricated evenly with a minimum
amount of lubricant.
• The pipette is reassembled improperly.
Disassemble the pipette and reassemble it, observ-
ing the proper sequence of steps (Fig. 3).
• No lubricant on the sealing elements.
Remove the tip ejector. Unscrew the shaft nut,
remove the shaft, piston assembly, seal and O-ring.
Rinse the removed parts in distilled water and dry
thoroughly. Lightly lubricate the inside surfaces of
the seal and the O-ring with the included lubricant.
Reassemble the pipette in the reverse order.
• Contamination of the inside of the pipette caused by
extensive aspiration of chemically aggre ssive liquids or
because liquid got inside the pipette.
Remove the tip ejector. Unscrew the nut, remove
the shaft, piston assembly, seal and O-ring. Rinse
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the removed parts with distilled water and dry thor-
oughly. Lightly lubricate the inside surfaces of the
seal and the O-ring with the lubricant. Reassemble
the pipette in the reverse order.
If you find an increase in the pipetting force, which
could happen after repetitive autoclaving of the pipette:
Remove the tip ejector. Unscrew the shaft nut, then
remove the shaft, piston assembly, seal and O-ring.
Rinse the removed parts in distilled water and dry.
Lubricate the internal surfaces of the seal and O-ring
with lubricant that has been included with each
pipette. Reassemble the pipette in opposite order.
Note: All parts of the pipette can be autoclaved (see
section 10)
The shaft of the 5000 and 10000 models should be
autoclaved without the filter.
If the problem continues after carrying out the above
steps, contact your HTL representative.
Before returning the pipette, please ensure that the
pipette is completely free of any chemical, radioactive or
microbiological contamination which could pose a threat
during transport and repair. As far as it is possible, clean
the pipette.
10 - CLEANING AND STERILIZATION
Cleaning
External surfaces of the pipetting pushbutton, the ejector
pushbutton, the handgrip, the shaft nut and the adjust-
ment knob may be cleaned using a cloth dampened in
isopropyl alcohol. The remaining parts removed from the
pipette during pipette disassembly may be washed with
distilled water or isopropyl alcohol.
Warning: Before using cleaning agents other than those
recommended by the manufacturer, check the compati-
bility charts and consider chemical resistance of the fol-
lowing plastics: PEI, PP, PC, POM, PA, PPS, PVDF used
to produce the pipette parts.
Sterilization:
The pipette can be sterilized in the autoclave at 121°C for
20 minutes. After sterilization, the pipette should be dried
and cooled to room temperature.
ENGLISH
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