Repetitive Reverse Technique; Recommendations For Good Pipetting - Biohit mLINE Mode D'emploi

8.3. Repetitive reverse technique

1. Follow the reverse technique steps 1 to 5.
2. Continue pipetting by repeating steps 3 to 5 as long as needed.
3. Finally discard the remaining liquid by pressing the operating
button to the second stop.
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Recommendations for good pipetting

• Make sure that the tip is firmly attached to the tip cone.
• Hold the pipettor vertically when aspirating the liquid and place the
tip only a few millimeters into the liquid.
• Always control the operating button slowly and smoothly.
• Pre-rinse the tip before aspirating the liquid by filling and emptying
the tip for three to five times. This is important especially when
pipetting liquids with a viscosity and density greater than water or
liquids with high vapor pressure (e.g. ethanol).
• Check that the pipettor, tip and liquid are at the same temperature.
• When pipetting liquids with temperatures different to the ambient
temperature change the tip after each pipetting. Do not pre-rinse
the tip.
• To avoid contamination, do not rest the pipettor on its side
especially with the tip attached.
• Change the tip cone filter regularly (recommendation after 50 – 250
pipetting cycles).
• Never strike the tip cone against the tip tray when mounting the tip
as this can damage the pipettor.
• Avoid rough handling and do not drop the pipettor.
• Avoid exposing the unit to extreme temperature changes, humidity
and dust (operating temperature from 15ºC to 40ºC).
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