Reverse Pipetting; Pipetting Recommendations - LABBOX EASY 40 Elite Mode D'emploi

4. Release the thumb button to the ready position (Fig.6A). If necessary
change the tip and continue with pipetting.
Starting position
Fig.6A

6.2. Reverse pipetting

The reverse technique is suitable for dispensing liquids that have a tendency to
foam or have a high viscosity. This technique is also used for dispensing very small
volumes when it is recommended that the tip is first primed with the liquid before
pipetting. This is achieved by filling and emptying the tip.
1. Depress the thumb button all the way to the second stop (Fig.6C). Place the
tip just under the surface of the liquid
(2-3mm) and smoothly release the thumb button.
2. Withdraw the tip from the liquid touching against the edge of the container to
remove excess.
3. Deliver the preset volume by smoothly depressing thumb button to the first
stop (Fig.6B). Hold the thumb button at the first stop. The liquid that remains
in the tip should not be included in the delivery.
4. The remaining liquid should now be discarded with the tip or delivered back
into the container vessel.

7. PIPETTING RECOMMENDATIONS

Hold the micropipette vertically when aspirating the liquid and place the only

a few millimeters into the liquid
Prerinse the tip before aspirating the liquid by filling and emptying the tip

5 times. This is important especially when dispensing liquids which have a
viscosity and density different from water
Always control the push button movements with the thumb to ensure

consistency
When pipetting liquids at a temperature different from ambient, prerinse the

tip several times before use.
8. STORAGE
When not in use it is recommended that your micropipette is stored in a vertical
position.
9. PERFORMANCE TEST AND RECALIBRATION
Each pipette has been factory-tested and certified at 22ºC according to ISO8655/
DIN12650. The following table shows the maximum permitted errors (F
manufacturers gives in ISO8655/DIN 12650, which further advises each user to
establish their own maximum permitted errors (F
exceed the F by more than 100%.
max
Note: Micropipette specifications are guaranteed only with manufacturer's tips.
9.1. Performance test (Checking calibration)
Weighting should take place at 20-25ºC, constant to + 0.5ºC.

Avoid drafts.

1. Set the desired testing volume of your micropipette.
2. Carefully fit tip onto the tip cone.
3. Prerinse tip with distilled water by pipetting the selected volume 5
times.
4. Carefully aspirate the liquid, keeping the micropipette vertical.
5. Pipette distilled water into a tared container read the weight in mgs.
Repeat at least five times and record each result. Use an analytical
balance with a readability of 0.01 mgs. To calculate the volume,
divide the weight of the water by its density (at 20ºC: 0.9982). This
method is based on ISO8655/DIN12650.
4
First stop
Fig.6B
user). The F
max
Second stop
Fig.6C
) for
max
user should not
max