Important Guidelines; Operation - VWR 700-0450 Manuel D'instructions

Important Guidelines

Important guidelines for operating the 300V Hi Amperage Power Supply are provided
in this section. We recommend that you carefully review these guidelines before
operating the instrument.
Important: For best results, do NOT use the 300V Hi Amperage Power Supply
at its maximum electrical load limits. Variations in buffer conditions can result in
exceeding the power supply's maximum voltage, current, or power output capacity
and produce undesirable variations in electrophoretic separations.
General Operating Instructions
Follow the instructions below to operate the 300V Hi Amperage Power Supply.
• Turn on the 300V Hi Amperage Power Supply by pressing the power switch on the
rear side of the instrument. Upon start-up, the Display Screen on the front of the
instrument will illuminate.
• Use the START/PAUSE and STOP keys to switch on and off the power to the output
jacks.
Recommendation
The duration of electrophoresis can be defined in time (hours/minutes). When using
this or any electrophoresis product, we recommend that you adhere to the time
protocols provided in protocol and application manuals.
For best results, follow these important guidelines when running
Important:
multiple gels and electrophoresis units concurrently.
• Avoid running samples with differing buffer salt concentrations at the same time.
Note: Variations in conductivity due to differences in buffer salt concentrations can
affect the run of all the samples run at the same time.

Operation

The 300 Volt Hi Amperage Power Supply is designed to operate under different
modes, Constant mode or Programming Mode, depending upon your
electrophoresis needs.
Use the Constant Voltage, Constant Power or Constant Current Operation for
applications that require only one specific voltage limit, current limit, and power limit
continuously during the entire duration of electrophoresis.
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