Leica BOND Mode D'emploi page 4

Precautions
• This product is intended for in vitro diagnostic use.
• The concentration of ProClin
the skin, eyes, mucous membranes and upper respiratory tract. Wear disposable gloves when handling reagents.
• To obtain a copy of the Material Safety Data Sheet contact your local distributor or regional office of Leica Biosystems, or
alternatively, visit the Leica Biosystems' Web site, www.LeicaBiosystems.com.
• Specimens, before and after fixation, and all materials exposed to them, should be handled as if capable of transmitting infection and
disposed of with proper precautions
reagents or specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek
medical advice.
• Consult Federal, State or local regulations for disposal of any potentially toxic components.
• Minimize microbial contamination of reagents or an increase in non-specific staining may occur.
• Retrieval, incubation times or temperatures other than those specified may give erroneous results. Any such change must be
validated by the user.
Instructions for Use
Myosin Heavy Chain (smooth muscle) (S131) primary antibody was developed for use on the automated BOND system in combination
with BOND Polymer Refine Detection. The recommended staining protocol for Myosin Heavy Chain (smooth muscle) (S131) primary
antibody is IHC Protocol F. Heat induced epitope retrieval is recommended using BOND Epitope Retrieval Solution 2 for 20 minutes.
Results Expected
Normal Tissues
Clone S131 detected the myosin heavy chain (smooth muscle) antigen in visceral and vascular smooth muscle, myoepithelial cells in
ducts of breast and in periacinar and periductal cells in salivary gland. (n=117).
Tumor Tissues
Clone S131 stained 5/5 leiomyosarcomas and 15/15 leiomyomas. No staining was observed in a variety of additional benign and
neoplastic tissues, including rhabdomyosarcomas (n=90).
Myosin Heavy Chain (smooth muscle) (S131) is recommended for use in detecting myosin heavy chain (smooth muscle)
protein expression in a variety of normal and neoplastic tissues.
Product Specific Limitations
Myosin Heavy Chain (smooth muscle) (S131) has been optimized at Leica Biosystems for use with BOND Polymer Refine Detection and
BOND ancillary reagents. Users who deviate from recommended test procedures must accept responsibility for interpretation of patient
results under these circumstances. The protocol times may vary, due to variation in tissue fixation and the effectiveness of antigen
enhancement, and must be determined empirically. Negative reagent controls should be used when optimizing retrieval conditions and
protocol times.
Troubleshooting
Refer to reference 3 for remedial action.
Contact your local distributor or the regional office of Leica Biosystems to report unusual staining.
Further Information
Further information on immunostaining with BOND reagents, under the headings Principle of the Procedure, Materials Required,
Specimen Preparation, Quality Control, Assay Verification, Interpretation of Staining, Key to Symbols on Labels, and General Limitations
can be found in "Using BOND Reagents" in your BOND user documentation.
Bibliography
1. Clinical Laboratory Improvement Amendments of 1988, Final Rule 57 FR 7163 February 28, 1992.
2. Villanova PA. National Committee for Clinical Laboratory Standards (NCCLS). Protection of laboratory workers from infectious
diseases transmitted by blood and tissue; proposed guideline. 1991; 7(9). Order code M29-P.
3. Bancroft JD and Stevens A. Theory and Practice of Histological Techniques. 4th Edition. Churchill Livingstone, New York. 1996.
4. Zhu L, Vranckx R, Khau Van Kien P, et al. Mutations in myosin heavy chain 11 cause a syndrome associating thoracic aortic
aneurysm/aortic dissection and patent ductus arteriosus. Nature Genetics. 2006; 38(3):343–349.
Date of Issue
10 September 2018
PA0493
Page 3
950 is 0.35%. It contains the active ingredient 2-methyl-4-isothiazolin-3-one, and may cause irritation to
TM
2 . Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with