Leica BOND Mode D'emploi page 3

BOND Ready-To-Use Primary Antibody
™
Myosin Heavy Chain (smooth muscle) (S131)
Catalog No: PA0493
Intended use
This reagent is for in vitro diagnostic use.
Myosin Heavy Chain (smooth muscle) (S131) monoclonal antibody is intended to be used for the qualitative identification by light
microscopy of human myosin heavy chain (smooth muscle) protein in formalin-fixed, paraffin-embedded tissue by immunohistochemical
staining using the automated BOND system (includes Leica BOND-MAX system and Leica BOND-III system).
The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and
should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Summary and Explanation
Immunohistochemical techniques can be used to demonstrate the presence of antigens in tissue and cells (see "Using BOND Reagents"
in your BOND user documentation). Myosin Heavy Chain (smooth muscle) (S131) primary antibody is a ready to use product that
has been specifically optimized for use with BOND Polymer Refine Detection. The demonstration of human myosin heavy chain
(smooth muscle) protein. is achieved by first, allowing the binding of Myosin Heavy Chain (smooth muscle) (S131) to the section, and
then visualizing this binding using the reagents provided in the detection system. The use of these products, in combination with the
automated BOND system, reduces the possibility of human error and inherent variability resulting from individual reagent dilution,
manual pipetting and reagent application.
Reagents Provided
Myosin Heavy Chain (smooth muscle) (S131) is a mouse anti-human monoclonal antibody produced as a tissue culture supernatant,
and supplied in Tris buffered saline with carrier protein, containing 0.35% ProClin 950 as a preservative.
TM
Total volume = 7 mL.
Clone
S131.
Immunogen
Prokaryotic recombinant fusion protein corresponding to a 235 amino acid region of the C-terminal domain of the human myosin-heavy
chain (smooth muscle) molecule.
Specificity
Human myosin heavy chain (smooth muscle) protein.
Subclass
IgG2b.
Total Protein Concentration
Approx 10 mg/mL.
Antibody Concentration
Greater than or equal to 0.28 mg/L as determined by ELISA.
Dilution and Mixing
Myosin Heavy Chain (smooth muscle) (S131) primary antibody is optimally diluted for use on the BOND system. Reconstitution, mixing,
dilution or titration of this reagent is not required.
Materials Required But Not Provided
Refer to "Using BOND Reagents" in your BOND user documentation for a complete list of materials required for specimen treatment and
immunohistochemical staining using the BOND system.
Storage and Stability
Store at 2–8 °C. Do not use after the expiration date indicated on the container label.
The signs indicating contamination and/or instability of Myosin Heavy Chain (smooth muscle) (S131) are: turbidity of the solution, odor
development, and presence of precipitate.
Return to 2–8 °C immediately after use.
Storage conditions other than those specified above must be verified by the user .
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PA0493
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