Optika Italy OPTISCAN10 Manuel D'utilisation page 8

STEP 6 – Scan / Exit
Select Scan to scan and save the file.
Once you have finished scanning Exit the program by clicking on
File on the menu then Close.
One Button Scan
1. Open the SciView (see above to acquire the SciView driver program)
2. Configure Scan Settings (see above)
3. Press the one touch scan button to begin the scan to file process.
Suggestions for Making Your Own Prepared Microscope Slides
Always make sure clean slides and cover glass are used.
Handle slides carefully by the edges to avoid fingerprints.
If you have forceps or tweezers, use it to carefully handle cover glass.
The thin cover glass will break if too much pressure is applied!
It is highly recommended to use a blade for cutting specimens. Keep the blade covered and store in a safe
place when not in use.
Making a Temporary "Wet" Mounted Slide
• Tissue sections:
1. Use the blade to carefully cut the specimen as thin as possible.
2. Place the specimen on the slide.
3. Use the plastic dropper to place one drop of water on the specimen. Note: Steps 2 & 3 can be reversed.
Find the best protocol for each specimen.
4. Carefully place a cover glass over the specimen, allowing the water to spread under the glass.
5. The cover slip should be flattened against the specimen and slide as much as possible, removing air
bubbles if any.
6. Blot any excess water carefully with paper tissues.
7. Carefully place slide on the scanner holder (cover glass side up) and scan.
8. Remember to properly name your scanned images in the SciView software.
• Tip:
1. Slides may be carefully washed and re-used for future specimens. Cover glass is extremely fragile and not
recommended for re-using. Carefully discard after use.
2. With scanner, you now have the ability to observe a wide range of different objects, such as tissue
sections, common things, and aquatic organisms. Below are some more ideas for observation.
3. Many specimens are transparent. You may try staining your tissue specimens in food color for a couple of
minutes before mounting it on the slide. Use the tweezers to pick up the "stained" specimen.
4. Scanning fresh specimens that are several-cells thick with pigments/colors, such as plant leaves and black
hair, will likely return with dark black images without showing any detail of the specimens.
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