Calibration - Thermo Electron FINNPIPETTE COLOUR MULTICHANNELS Guide D'utilisation

ENGLISH
5. Clean the pistons and O-rings with a dry napless cloth
and grease them slightly with grease (included in the
package).
6. Reassemble the pipette.
In the event of a malfunction clean the pipette and
send it to the Thermo Electron representative.
Please, enclose a complete list of liquids which have
entered the pipette.
All Finnpipettes are factory calibrated and adjusted to give
the volumes as specified with distilled or deionized water.
The pipettes are constructed to permit re-adjustment for
liquids of different temperature and viscosity.
DEVICE REQUIREMENTS AND TEST CONDITIONS
An analytical balance must be used. The scale graduation
value of the balance should be chosen according to the
selected test volume of the pipette:
Volume range
under 10 µl
10-100 µl
above 100 µl
Test liquid: Water, distilled or deionized, "grade 3" water
conforming ISO 3696. Tests are done in a draft-free room
at a constant (±0.5°C) temperature of water, pipette and
air between 15°C to 30°C.
The relative humidity must be above 50%. Especially with
volumes under 50 µl the air humidity should be as high as
possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are
recommended.
Procedure to check calibration
The pipette is checked with the maximum volume (nominal
volume) and with the minimum volume. A new tip is first
pre-wetted 3-5 times and a series of ten pipettings is done
with both volumes. A pipette is always adjusted for
delivery (Ex) of the selected volume.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of
both series.
4. Compare the results to the limits in the Table 1.
If the calculated results are within the selected limits, the
adjustment of the pipette is correct.
TABLE 1: Maximum permissible errors according ISO8655

CALIBRATION

readable graduation
0.00 1 mg
0.01 mg
0.1 mg
6