Operation Of Integrated Modulation Contrast (Imc) - Leica DMIL Mode D'emploi

Operation of
Integrated Modulation Contrast (IMC)
Integrated Modulation Contrast is a special form
of oblique illumination based on the principle of
Hoffman's modulation contrast.
With this method, a modulator is used to convert
the phase gradients of an unstained object into
amplitude differences.
The impression of a three-dimensional image is
created, similar to a microscopic image with
interference contrast. However, other than with
interference contrast, the object can also be
viewed through double-refracting plastic mate-
rials, such as Petri dishes.
Additional benefits of this imaging method
include:
– High contrast
– High resolution
– Halo-free, variable-contrast relief image
– Long working distance of the condenser
– Simple assembly and adjustment
– Applicable for stained and unstained
specimens
Important!
IMC is only possible in conjunction with the S 55
condenser.
Standard bright-field and phase contrast objec-
tives can be used for IMC, which permits to
cover the magnification range from 5x to 100x.
The following objectives are especially suited:
C PLAN 10x/0.22 AP 32.2
C PLAN L 20x/0.30 D
C PLAN L 40x/0.50 D
plus the corresponding phase contrast
objectives.
All other objectives with pupil position D can
also be used.
The following objectives with pupil position C
may also be used with some restrictions:
N PLAN L 20x/0.40 Corr
N PLAN L 40x/0.55 Corr
®
PL FLUOTAR L 63x/0.70 Corr
(Refer also to Possible errors).
The IMC requires the use of the IMC modulator
(49.1) and the IMC slit-diaphragm slide (49.2).
Positioning the IMC modulator
• Remove the empty slide in the microscope
stand if present.
• Position the IMC modulator so that the
lettering points forward.
• Lock the slide in position IMC (lettering IMC
visible).
The IMC modulator will be flush on either side.
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