Phase Contrast; Darkfield - Leica DM1000 Mode D'emploi

9. Contrast Methods

9.1.2 Phase Contrast

• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
Objectives that are suitable for phase contrast
are engraved with PH.
• Bring the image into focus using the focus dial
and set the brightness.
• For an optimal field diaphragm setting, check
the Köhler illumination (→ p. 2831
• Open the aperture diaphragm
(position PH).
• Condensers UCL/UCLP and UCA/P:
Set the light ring corresponding to the
objective on the condenser disk.
Example: Light ring 1 belongs to the objective
with the engraving PH 1.
Condensers CL/PH,
ACHR.0.9 (P):
Use the light ring slide.
Note:
Condensers UCL/UCLP and UCA/P: Light rings
must be centered (→ p. 29).
44
completely
CLP/PH and APL.

9.1.3 Darkfield

• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
• Bring the image into focus using the focus dial
and set the brightness.
• Condenser UCL/P:
Click the condenser disk into the BF position.
Condensers CL/PH,
ACHR.0.9 (P):
Pull out the DF light ring slide as far as the
stop.
Check the Köhler illumination (→ p. 28).
• Open the aperture diaphragm
(position PH).
• Condenser UCL/P:
Click the condenser disk into the DF position.
Condensers CL/PH,
ACHR.0.9 (P):
Insert the DF light ring slide as far as the stop.
Note:
Condensers UCL/UCLP and UCA/P: The DF light
ring must be centered (→ p. 29).
Special dark field condensers are available for
the DM1000 (Fig. 61).
The application potential of the DF condensers
depends on the aperture of the objective in use.
For objectives with built-in iris diaphragm, the
aperture can be adapted.
DF condenser
D 0.80 - 0.95
D 1.20 - 1.44 OIL
CLP/PH und
completely
CLP/PH und
max. objective aperture
0.75
1.10
APL.
APL.